IL-35 Protein, Human, Recombinant

Catalog No. TMPY-03879

IL-35 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 102 kDa and the accession number is P29459-1.

IL-35 Protein, Human, Recombinant

Catalog No. TMPY-03879

IL-35 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 102 kDa and the accession number is P29459-1.

Pack Size	Price	Availability	Quantity
50 μg	$618	7-10 days
500 μg	$2,510	7-10 days

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User Guide of Recombinant Proteins

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Product Information

Biological Activity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	IL-35 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 102 kDa and the accession number is P29459-1.
Species	Human
Expression System	HEK293 Cells
Tag	Tag Free
Accession Number	P29459-1
Synonyms	P35,NKSF1,NFSK,Interleukin-35,interleukin 12A,IL-35,IL-12A,CLMF
Construction	A DNA sequence encoding the p35 subunit of human IL12, termed as IL12A (P29459-1) (Met1-Ser219) was fused with a C-terminal Fc region of human IgG1 tag followed by a His tag, constructed the plasmid 1; A DNA sequence encoding the human IL27B, (NP_005746.2) (Met1-Lys229) was fused with a C-terminal Fc region of human IgG1 tag followed by a FLAG tag, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. Predicted N terminal: Arg 23 & Arg 21
Protein Purity	> 90 % as determined by SDS-PAGE
Molecular Weight	102 kDa (predicted); 60 & 63 kDa (reducing conditions)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	IL12A is a subunit of a cytokine that acts on T and natural killer cells and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. IL12A, together with IL27B, form a disulfide-linked heterodimer: IL12A&IL27B. IL12A&IL27B is required for the T-cell-independent induction of IFN-gamma and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of IL12A&IL27B in innate immunity.