Shopping Cart

Remove All

Your shopping cart is currently empty

IL-6 Protein, Human, Recombinant

Resource Download

COA

IL-6 Protein, Human, Recombinant

Catalog No. TMPY-00863

IL-6 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 20.95 kDa and the accession number is P05231.

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Pack Size	Price	Availability
20 μg	$177	In Stock
100 μg	$447	7-10 days
200 μg	$698	7-10 days
500 μg	$1,950	7-10 days

Bulk & Custom

Add to Cart

Questions

Biological Description

Biological Information	1. Immobilized Human IL-6 at 2 μg/mL (100 μL/well) can bind Human IL-6R hFc, the EC50 of Human IL-6R hFc is 3.0-10.0 ng/mL. 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.1-0.8 ng/mL. 3.Loaded Recombinant Human IL-6R Protein, His Tag on His1K Biosensor, can bind Recombinant Human IL-6 Protein, no Tag with an affinity constant of 0.165 µM as determined in BLI assay (Routinely tested).
Description	IL-6 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 20.95 kDa and the accession number is P05231.
Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	P05231
Synonyms	BSF2,HSF,IL-6,IFNB2,interleukin 6,HGF,Interleukin-6
Construction	The mature form of Human IL6 (NP_000591.1) (Val30-Met212) was expressed with an initial Met at the N-terminus.
Protein Purity	≥ 95 % as determined by SDS-PAGE
Molecular Weight	20.95 kDa (predicted)
Endotoxin	< 10 EU/mg of the protein.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as a phosphorylated and variably glycosylated molecule. It exerts actions through its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined as an essential role in directing the transition from innate to acquired immunity.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy