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IL-6 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 20.95 kDa and the accession number is P05231.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μg | $177 | In Stock | |
100 μg | $447 | 7-10 days | |
200 μg | $698 | 7-10 days | |
500 μg | $1,950 | 7-10 days |
Biological Activity | 1. Immobilized Human IL-6 at 2 μg/mL (100 μL/well) can bind Human IL-6R hFc, the EC50 of Human IL-6R hFc is 3.0-10.0 ng/mL._x000D_
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.1-0.8 ng/mL._x000D_
3.Loaded Recombinant Human IL-6R Protein, His Tag on His1K Biosensor, can bind Recombinant Human IL-6 Protein, no Tag with an affinity constant of 0.165 µM as determined in BLI assay (Routinely tested). |
Description | IL-6 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 20.95 kDa and the accession number is P05231. |
Species | Human |
Expression System | E. coli |
Tag | Tag Free |
Accession Number | P05231 |
Synonyms | Interleukin-6,interleukin 6,IL-6,IFNB2,HSF,HGF,BSF2 |
Construction | A DNA sequence encoding the mature form of human IL6 (NP_000591.1) (Val30-Met212) was expressed with an initial Met at the N-terminus. Predicted N terminal: Met |
Protein Purity | ≥ 95 % as determined by SDS-PAGE |
Molecular Weight | 20.95 kDa (predicted); 19.7 kDa (reducing conditions) |
Endotoxin | < 10 EU/mg of the protein. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as a phosphorylated and variably glycosylated molecule. It exerts actions through its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined as an essential role in directing the transition from innate to acquired immunity.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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