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Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1/M1 Protein (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 29.3 kDa and the accession number is B4UPA8.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $517 | In Stock | |
200 μg | $883 | 7-10 days | |
500 μg | $1,780 | 7-10 days |
Biological Activity | Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
Description | Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1/M1 Protein (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 29.3 kDa and the accession number is B4UPA8. |
Species | H1N1 |
Expression System | E. coli |
Tag | N-His |
Accession Number | B4UPA8 |
Synonyms | cholinergic receptor, muscarinic 1 |
Construction | A DNA sequence encoding the influenza A H1N1 Virus (A/Puerto Rico/8/34/Mount Sinai) M1 protein (NP_040978.1) (Ser 2-Lys 252) was expressed, with a polyhistidine tag at the N-terminus. Predicted N terminal: Met |
Protein Purity | > 90 % as determined by SDS-PAGE |
Molecular Weight | 29.3 kDa (predicted); 29 kDa (reducing conditions) |
Endotoxin | Please contact us for more information. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM tris, 0.5 mM DTT, 0.5 mM EDTA, 5% glycerol, 50 mM NaCl, pH 7.6. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | The Influenza virus matrix protein 1 (M1) is a matrix protein of the influenza virus. M1 protein has been shown to play a crucial role in virus replication, assembly, and budding. It forms a coat inside the viral envelope. This is a bifunctional membrane/RNA-binding protein that mediates the encapsidation of RNA-nucleoprotein cores into the membrane envelope. M1 consists of two domains connected by a linker sequence. The N-terminal domain has a multi-helical structure. The C-terminal domain also contains an alpha-helical structure. The M1 protein is the most abundant structural protein in influenza A virus particles. M1 protein of the influenza A virus plays multiple roles in virion assembly and infection. M1 protein was a candidate antigen for a broad-spectrum influenza virus vaccine and the adjuvant chitosan significantly improved the efficacy of the M1 vaccine. |
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