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Influenza A H1N1 (A/Brevig Mission/1/1918) Matrix protein 1/M1 Protein (His)

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Influenza A H1N1 (A/Brevig Mission/1/1918) Matrix protein 1/M1 Protein (His)

Catalog No. TMPY-00496

Influenza A H1N1 (A/Brevig Mission/1/1918) Matrix protein 1/M1 Protein (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 30 kDa and the accession number is Q8BAC3.1.

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100 μg	$801	7-10 days

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Biological Description

Biological Information	Testing in progress
Description	Influenza A H1N1 (A/Brevig Mission/1/1918) Matrix protein 1/M1 Protein (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 30 kDa and the accession number is Q8BAC3.1.
Species	H1N1
Expression System	E. coli
Tag	N-His
Accession Number	Q8BAC3.1
Synonyms	cholinergic receptor, muscarinic 1
Construction	The influenza A virus (A/Brevig Mission/1/ 1918(H1N1)) matrix protein 1(Q8BAC3.1) (Met1-Lys252), termed as M1, was fused with a N-terminal polyhistidine tag.
Protein Purity	> 90 % as determined by SDS-PAGE
Molecular Weight	30 kDa (predicted)
Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 1 mM EDTA, 50 mM Tris, 50 mM NaCl, 5% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The Influenza virus matrix protein 1 (M1) is a matrix protein of the influenza virus. M1 protein has been shown to play a crucial role in virus replication, assembly, and budding. It forms a coat inside the viral envelope. This is a bifunctional membrane/RNA-binding protein that mediates the encapsidation of RNA-nucleoprotein cores into the membrane envelope. M1 consists of two domains connected by a linker sequence. The N-terminal domain has a multi-helical structure. The C-terminal domain also contains an alpha-helical structure. The M1 protein is the most abundant structural protein in influenza A virus particles. M1 protein of the influenza A virus plays multiple roles in virion assembly and infection. M1 protein was a candidate antigen for a broad-spectrum influenza virus vaccine and the adjuvant chitosan significantly improved the efficacy of the M1 vaccine.