KIR3DL3 Protein, Human, Recombinant (His & Avi)

Catalog No. TMPK-00256

Killer-cell immunoglobulin-like receptor (KIR) 3DL3 is a framework gene present in all human KIR haplotypes.KIR3DL3 has been shown to be constitutively expressed at a low RNA level in peripheral blood mononuclear cell (PBMC) and decidual natural kill (NK) cells. KIR3DL3 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 35.3 kDa and the accession number is Q8N743.

KIR3DL3 Protein, Human, Recombinant (His & Avi)

Catalog No. TMPK-00256

Pack Size	Price	Availability
100 μg	$465	7-10 days
500 μg	$1,850	7-10 days
1 mg	$3,100	7-10 days

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Product Information

Biological Activity	Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Human KIR3DL3, His Tag with an affinity constant of 334 nM as determined in SPR assay.
Description	Killer-cell immunoglobulin-like receptor (KIR) 3DL3 is a framework gene present in all human KIR haplotypes.KIR3DL3 has been shown to be constitutively expressed at a low RNA level in peripheral blood mononuclear cell (PBMC) and decidual natural kill (NK) cells. KIR3DL3 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 35.3 kDa and the accession number is Q8N743.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	Q8N743
Synonyms	KIRC1,KIR44,KIR3DL7,KIR3DL3,KIR2DS2,CD158Z
Construction	Glu26-Leu322
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	35.3 kDa (predicted). Due to glycosylation, the protein migrates to 48-52 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 150 mM NaCl, 0.2M L-arginine (pH 8.2). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Killer-cell immunoglobulin-like receptor (KIR) 3DL3 is a framework gene present in all human KIR haplotypes.KIR3DL3 has been shown to be constitutively expressed at a low RNA level in peripheral blood mononuclear cell (PBMC) and decidual natural kill (NK) cells.