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Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $511 | 7-10 days | |
500 μg | $2,040 | 7-10 days | |
1 mg | $3,410 | 7-10 days |
Description | LY75/CD205 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 189.5 kDa and the accession number is Q60767. |
Species | Mouse |
Expression System | HEK293 Cells |
Tag | C-His |
Accession Number | Q60767 |
Synonyms | CLEC13B,GP200-MR6,DEC-205,Ly-75,CD205 |
Construction | Ser28-Asp1667 |
Protein Purity | > 95% as determined by Tris-Bis PAGE |
Molecular Weight | 189.5 kDa (predicted). Due to glycosylation, the protein migrates to 235-250 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | Lymphocyte antigen 75 (Ly75/CD205) is a surface marker on mitotic germ cells in rainbow trout. In mammals, several cell surface molecular markers have been characterized in order to identify the mitotic germ cells. he expression profile of Ly75 protein was similar to that of the mRNA. Furthermore, identification of various fish homologs of ly75 confirmed that their amino acid sequences are well conserved. Therefore, Ly75 may be appropriate for use as a versatile cell surface marker for mitotic germ cells in fish. |
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