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Periostin/OSF-2 Protein, Human, Recombinant (aa 22-836, His)

Catalog No. TMPK-00973

Periostin/OSF-2 Protein, Human, Recombinant (aa 22-836, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 92.1 kDa and the accession number is Q15063.

Periostin/OSF-2 Protein, Human, Recombinant (aa 22-836, His)

Periostin/OSF-2 Protein, Human, Recombinant (aa 22-836, His)

Catalog No. TMPK-00973
Periostin/OSF-2 Protein, Human, Recombinant (aa 22-836, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 92.1 kDa and the accession number is Q15063.
Pack SizePriceAvailabilityQuantity
100 μg$5117-10 days
500 μg$2,0407-10 days
1 mg$3,4107-10 days
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Product Information

Biological Activity
Immobilized Human Periostin, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA.
Description
Periostin/OSF-2 Protein, Human, Recombinant (aa 22-836, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 92.1 kDa and the accession number is Q15063.
Species
Human
Expression System
HEK293 Cells
TagC-His
Accession NumberQ15063
Synonyms
TRIF52,POSTN,PNRP11-412K4.1,PN,Periostin,PDLPOSTN,OSF-2,OSF2,Fasciclin I-like
Construction
Asn22-Gln836
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight92.1 kDa (predicted). Due to glycosylation, the protein migrates to 93-100 kDa based on Tris-Bis PAGE result.
Endotoxin< 1 EU/μg by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
Periostin is a matricellular protein that is expressed in several tissues during embryonic development; however, its expression in adults is mostly restricted to collagen-rich connective tissues. Periostin is expressed only briefly during kidney development, but it is not normally detected in the adult kidney. Recent evidence has revealed that periostin is aberrantly expressed in several forms of chronic kidney disease (CKD), and that its expression correlates with the degree of interstitial fibrosis and the decline in renal function.

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