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Periostin/OSF-2 Protein, Human, Recombinant (aa 22-836, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 92.1 kDa and the accession number is Q15063.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $511 | 7-10 days | |
500 μg | $2,040 | 7-10 days | |
1 mg | $3,410 | 7-10 days |
Biological Activity | Immobilized Human Periostin, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA. |
Description | Periostin/OSF-2 Protein, Human, Recombinant (aa 22-836, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 92.1 kDa and the accession number is Q15063. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-His |
Accession Number | Q15063 |
Synonyms | TRIF52,POSTN,PNRP11-412K4.1,PN,Periostin,PDLPOSTN,OSF-2,OSF2,Fasciclin I-like |
Construction | Asn22-Gln836 |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Molecular Weight | 92.1 kDa (predicted). Due to glycosylation, the protein migrates to 93-100 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | Periostin is a matricellular protein that is expressed in several tissues during embryonic development; however, its expression in adults is mostly restricted to collagen-rich connective tissues. Periostin is expressed only briefly during kidney development, but it is not normally detected in the adult kidney. Recent evidence has revealed that periostin is aberrantly expressed in several forms of chronic kidney disease (CKD), and that its expression correlates with the degree of interstitial fibrosis and the decline in renal function. |
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