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Periostin/OSF-2 Protein, Mouse, Recombinant (His)

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Periostin/OSF-2 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-01613

Periostin/OSF-2 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 89 kDa and the accession number is Q62009-2.

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Pack Size	Price	Availability
100 μg	$386	In Stock
200 μg	$660	7-10 days
500 μg	$1,340	7-10 days

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Biological Description

Biological Information	Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. When cells are added to POSTN-His coated plates (10 μg/mL, 100 μL/well), approximately >40% will adhere specifically after 30 minutes at 37℃.
Description	Periostin/OSF-2 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 89 kDa and the accession number is Q62009-2.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q62009-2
Synonyms	A630052E07Rik,OSF-2,Osf2,PLF,AI747096,peri,PN,periostin, osteoblast specific factor
Construction	The full length of Mouse POSTN (NP_056599.1) (Met 1-Gln 811) was expressed, with a polyhistidine tag at the C-terminus.
Protein Purity	> 92 % as determined by SDS-PAGE
Molecular Weight	89 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Periostin ( POSTN ), also known as OSF2 (osteoblast specific factor 2), is a heterofunctional secreted extracellular matrix (ECM) protein comprised of four fasciclin domains that promotes cellular adhesion and movement, as well as collagen fibrillogenesis. Postn is expressed in unique growth centers during embryonic development where it facilitates epithelial-mesenchymal transition (EMT) of select cell populations undergoing reorganization. In the adult, Postn expression is specifically induced in areas of tissue injury or areas with ongoing cellular re-organization. In the adult heart Postn is induced in the ventricles following myocardial infarction, pressure overload stimulation, or generalized cardiomyopathy. Although the detailed function of Postn is still unclear, Postn-integrin interaction is thought to be involved in tumor development. Postn is frequently overexpressed in various types of human cancers, stimulating metastatic growth by promoting cancer cell survival, invasion and angiogenesis, and can be a useful marker to predict the behavior of cancer.