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PVRIG Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 40.15 kDa and the accession number is Q6DKI7-1.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $600 | 7-10 days | |
1 mg | $3,910 | 7-10 days |
Biological Activity | 1.Measured by its binding ability in a functional ELISA. Immobilized Human Nectin-2 His at 2 μg/mL (100 μL/well) can bind Human PVRIG mFc, the EC50 of Human PVRIG mFc is 40-260 ng/mL.
2.Loaded Human PVRIG Protein, mFc Tag on AMC Biosensor, can bind Human Nectin-2 protein with an affinity constant of 0.680μM as determined in BLI assay (Routinely tested).
3.Loaded Human PVRIG Protein, mFc Tagon AMC Biosensor, can bind Human Nectin-2 protein, hFc Tag with an affinity constant of 8.784nM as determined in BLI assay (Routinely tested). |
Description | PVRIG Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 40.15 kDa and the accession number is Q6DKI7-1. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-mFc |
Accession Number | Q6DKI7-1 |
Synonyms | RMO1,RalGDS/AF-6,PREL-2,PREL2,LPD,C7orf15,ALS2CR9,ALS2CR18 |
Construction | A DNA sequence encoding the human PVRIG (Q6DKI7-1) (Thr41-Leu172) was expressed with the Fc region of mouse IgG2a at the C-terminus. Predicted N terminal: Thr 41 |
Protein Purity | > 90 % as determined by SDS-PAGE. |
Molecular Weight | 40.15 kDa (predicted); 52.66 kDa (reducing conditions) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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