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R-Spondin 3/RSPO3 Protein, Human, Recombinant (aa 33-272, His) is expressed in HEK293 Cells. The accession number is Q9BXY4-1.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μg | $275 | Backorder | |
100 μg | $695 | Backorder | |
1 mg | $3,520 | Backorder |
Biological Activity | Immobilized R-Spondin 3/RSPO3 Protein, Human, Recombinant (aa 33-272, His) (Cat#TMPY-07057) at 2 μg/mL (100 μL/well) can bind RNF43 Protein, Human, Recombinant (hFc) (Cat#TMPY-04149), the EC50 is 4-15 ng/mL. |
Description | R-Spondin 3/RSPO3 Protein, Human, Recombinant (aa 33-272, His) is expressed in HEK293 Cells. The accession number is Q9BXY4-1. |
Species | Human |
Expression System | HEK293 Cells |
Tag | N-His |
Accession Number | Q9BXY4-1 |
Synonyms | THSD2,PWTSR,FLJ14440,CRISTIN1 |
Construction | A DNA sequence encoding the Human RSPO3 (Q9BXY4-1) (Met33-His272) was expressed, with a polyhistidine tag at the N-terminus. |
Protein Purity | ≥ 95% as determined by SDS-PAGE. ≥ 95% as determined by SEC-HPLC. |
Molecular Weight | 29.3 kDa (predicted); 43.3 kDa (reducing contition, due to glycosylation) |
Endotoxin | < 50 EU per mg protein. |
Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
Reconstitution | Please refer to the lot-specific COA. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | R-spondin 3 (RSPO3) is a member of the R-Spondin (RSPO) family in vertebrates that activate Wnt/beta-catenin signaling, plays a key role in these processes. The RSPO family of secreted Wnt modulators is involved in development and disease and holds therapeutic promise as stem cell growth factors. The four members have high structural homology. RSPO2 and RSPO3 are more potent than RSPO1, whereas RSPO4 is relatively inactive. All RSPO members require Wnt ligands and LRP6 for activity and amplify signaling of Wnt3A, Wnt1, and Wnt7A, suggesting that RSPO proteins are general regulators of canonical Wnt signaling. RSPO3/PCP signaling during gastrulation requires Wnt5a and is transduced via Fz7, Dvl, and JNK. RSPO3 functions by inducing Sdc4-dependent, clathrin-mediated endocytosis. RSPO3 is a novel, evolutionarily conserved angiogenic factor in embryogenesis. RSPO3 has a key role in the interaction between chorion and allantois in labyrinthine development. |
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