1. Measured by its ability to induce activation of ßcatenin response in a_x000D_ Topflash Luciferase assay using HEK293T human embryonic kidney cells. The_x000D_ ED50 for this effect is typically 10-80 ng/mL in the presence of 5 ng/mL_x000D_ recombinant mouse Wnt3a.
_x000D_ 2. Human intestinal cancer organoids were cultured with RSPO1, EGF, NOG._x000D_ (Routinely tested).
_x000D_ 3. Human colorectum organoids were cultured with RSPO1, EGF, NOG._x000D_ (Routinely tested).
_x000D_ 4. Human lung cancer organoids were cultured with FGF2, FGF4, FGF7, EGF,_x000D_ FGF10, NOG, RSPO1. (Routinely tested).
_x000D_ 5. Human lung organoids were cultured with FGF2, FGF4, FGF7,EGF, FGF10,_x000D_ NOG, RSPO1. (Routinely tested).
_x000D_ 6. Human cholangiocarcinomas organoids were cultured with FGF2, HGF,_x000D_ FGF7, EGF, FGF10, NOG, RSPO1. (Routinely tested).
_x000D_ 7. Human liver cancer organoids were cultured with FGF2, HGF, FGF7, EGF,_x000D_ FGF10, TGFB1, NOG,RSPO1. (Routinely tested).
_x000D_ 8. Human kidney cancer organoids were cultured with FGF2, FGF7, EGF,_x000D_ FGF10, NOG, RSPO1. (Routinely tested).
_x000D_ 9. Human kidney organoids were cultured with FGF7, EGF, FGF10,NOG,_x000D_ RSPO1, HGF, FGF4. (Routinely tested).
_x000D_ 10. Human gastric cancer organoids were cultured with EGF, FGF10, NOG,_x000D_ RSPO1. (Routinely tested).
_x000D_ 11. Human stomach organoids organoids were cultured with EGF, FGF10,_x000D_ NOG, RSPO1. (Routinely tested).
_x000D_ 12. Human breast cancer organoids were cultured with FGF7, RSPO1, IGF1,_x000D_ EGF, NRG1 Beta 1, NOG. (Routinely tested).
_x000D_ 13. Human ovarian organoids were cultured with IGF1, NRG1 Beta 1,RSPO1,_x000D_ EGF, NOG. (Routinely tested).

R-Spondin 1/RSPO1 Protein, Human, Recombinant is expressed in CHO mammalian cells. The predicted molecular weight is 26.8 kDa and the accession number is Q2MKA7-1.

Human

CHO Cells

R-spondin 1,RSPO,CRISTIN3

A DNA sequence encoding the human RSPO1 (NP_001033722.1) (Met1-Ala263) was expressed and purified. Predicted N terminal: Ser 21

≥ 95 % as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.