R-Spondin 2/RSPO2 Protein, Human, Recombinant (hFc)

Catalog No. TMPY-06418

R-Spondin 2/RSPO2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 48.01 kDa and the accession number is Q6UXX9-1.

R-Spondin 2/RSPO2 Protein, Human, Recombinant (hFc)

Catalog No. TMPY-06418

R-Spondin 2/RSPO2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 48.01 kDa and the accession number is Q6UXX9-1.

Pack Size	Price	Availability
100 μg	$450	In Stock
200 μg	$795	7-10 days
500 μg	$1,680	7-10 days

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Product Information

Biological Activity	Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 2-12 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
Description	R-Spondin 2/RSPO2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 48.01 kDa and the accession number is Q6UXX9-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	Q6UXX9-1
Synonyms	R-spondin 2,CRISTIN2
Construction	A DNA sequence encoding the Human RSPO2 (Q6UXX9-1, with mutations L186P) (Met1-Gly205) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Gln 22
Protein Purity	> 90 % as determined by SDS-PAGE
Molecular Weight	48.01 kDa (predicted); 56.15 kDa (reducing conditions)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing Histidine, Arginine, 120 mM NaCl, 0.02% Tween 80, 5% sucrose, pH 6. 0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	R-spondin-2, also known as RSPO2, synergizes with Wnt to activate beta-catenin. RSPO2 is secreted proteins that regulate beta-catenin signaling. Activator of the beta-catenin signaling cascade leads to TCF-dependent gene activation. Action both in the canonical Wnt / beta- catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled / LRP receptors. Probably also acts as a ligand for frizzled and LRP receptors. The encoding gene Rspo2 was identified as a novel common integration site for the mouse mammary tumor virus in viral induced mouse mammary tumors. Rspo2 and Rspo2 / Wnt1 tumors contained many spindle cells, consistent with an epithelial-mesenchymal transformation phenotype. When Rspo2 and Rspo2 / Wnt1 tumor cells were transferred into naive mice, they exhibited greater metastatic activity than cells derived from Wnt1 tumors.