SHH Protein, Mouse, Recombinant (C25II)

Catalog No. TMPJ-01171

SHH Protein, Mouse, Recombinant (C25II) is expressed in E. coli expression system. The predicted molecular weight is 18-20 KDa and the accession number is Q62226.

SHH Protein, Mouse, Recombinant (C25II)

Catalog No. TMPJ-01171

SHH Protein, Mouse, Recombinant (C25II) is expressed in E. coli expression system. The predicted molecular weight is 18-20 KDa and the accession number is Q62226.

Pack Size	Price	Availability
10 μg	$129	7-10 days
50 μg	$390	7-10 days
500 μg	$1,900	7-10 days
1 mg	$2,730	7-10 days

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COA User Guide of Recombinant Proteins

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Product Information

Biological Activity	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	SHH Protein, Mouse, Recombinant (C25II) is expressed in E. coli expression system. The predicted molecular weight is 18-20 KDa and the accession number is Q62226.
Species	Mouse
Expression System	E. coli
Tag	Tag Free
Accession Number	Q62226
Synonyms	Sonic Hedgehog Protein,SHH,HHG-1
Amino Acid	Cys25-Gly198(Cys25Ile-Ile)
Construction	Cys25-Gly198(Cys25Ile-Ile)
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight	18-20 KDa (reducing condition)
Endotoxin	< 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 150 mM NaCl, 1 mM DTT, pH 7.4.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Mouse Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. The mature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa Nterminal fragment (ShhN), and a catalyticprocessing,glycosylated, 34 kDa, 239 aa Cterminal fragment. The 20 kDa ShhN fragment is the core of the active hedgehog molecule. Mouse ShhN is 99%, 98%, and 100% aa identical to human, rat and gerbil ShhN, respectively.