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SHH Protein, Mouse, Recombinant

Catalog No. TMPJ-01170

SHH Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 20 KDa and the accession number is Q62226.

SHH Protein, Mouse, Recombinant

SHH Protein, Mouse, Recombinant

Catalog No. TMPJ-01170
SHH Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 20 KDa and the accession number is Q62226.
Pack SizePriceAvailabilityQuantity
10 μg$1297-10 days
50 μg$3907-10 days
500 μg$1,9007-10 days
1 mg$2,7307-10 days
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Product Information

Biological Activity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
SHH Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 20 KDa and the accession number is Q62226.
Species
Mouse
Expression System
E. coli
TagTag Free
Accession NumberQ62226
Synonyms
Sonic Hedgehog Protein,SHH,HHG-1
Amino Acid
Cys25-Gly198
Construction
Cys25-Gly198
Protein Purity
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight20 KDa (reducing condition)
Endotoxin< 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background
Mouse Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. The mature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N­terminal fragment (Shh­N), and a catalytic­processing,glycosylated, 34 kDa, 239 aa C­terminal fragment. The 20 kDa Shh­N fragment is the core of the active hedgehog molecule. Mouse Shh­N is 99%, 98%, and 100% aa identical to human, rat and gerbil Shh­N, respectively.

Dose Conversion

You can also refer to dose conversion for different animals. More

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Please read the User Guide of Recombinant Proteins for more specific information.

Keywords