1. Loaded Human Siglec-10-Fc-Avi on SA Biosensor, can bind Anti-Human Siglec-10 mAb-Fc with an affinity constant of <10^-3 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Anti-Human Siglec10 mAb at 1μg/ml (100 μl/well) can bind Biotinylated Human Siglec-10-Fc-Avi The ED50 of Biotinylated Human Siglec-10-Fc-Avi is 1.45 ng/ml. (Regularly tested)

Siglec-10 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-Fc-Avi tag. The predicted molecular weight is 100-130 KDa and the accession number is Q96LC7.

Human

HEK293 Cells

SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7,SLG2,Siglec-like protein 2,siglec-like gene 2,Siglec-10,SIGLEC10,sialic acid-binding Ig-like lectin 10,PRO940,MGC126774

Met17­Thr546

Met17­Thr546

Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. Siglec-G is the apparent ortholog of human Siglec-10. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides. This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell. When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.