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Siglec-10 Protein, Human, Recombinant (R119A, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 62.64 kDa and the accession number is Q96LC7-1.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | 773 € | 7-10 days | |
500 μg | 3.087 € | 7-10 days | |
1 mg | 5.168 € | 7-10 days |
Biological Activity | Immobilized Biotinylated Human Siglec-10 (R119A) , His Tag at 2μg/ml (100μl/Well) on the streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. |
Description | Siglec-10 Protein, Human, Recombinant (R119A, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 62.64 kDa and the accession number is Q96LC7-1. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-His-Avi |
Accession Number | Q96LC7-1 |
Synonyms | SLG2,SIGLEC10,PRO940,MGC126774 |
Construction | Met17-Thr546 |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Molecular Weight | 62.64 kDa (predicted). Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tirs 150 mM NaCl, 200 mM L-arginine (pH 8.0). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. |
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