1. Loaded Human IL-33 on AR2G Biosensor, can bind Mouse ST2-Fc with an affinity constant of 2.28nM as determined in BLI assay. (Regularly tested) 2. Immobilized Mouse IL-33 at 5μg/ml (100 μl/well) can bind Mouse ST2-Fc. The ED50 of Mouse ST2-Fc is 0.25 ug/ml. (Regularly tested)

ST2/IL-1 RL1 Protein, Mouse, Recombinant (V192A, hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 90-110 KDa and the accession number is P14719-2.

Mouse

HEK293 Cells

suppression of tumorigenicity 2,ST2V,ST2L,Protein T1,Protein ST2,Lymphocyte antigen 84,Ly84,Interleukin-1 receptor-like 1,IL-33R,IL-1RL1,Il1rl1,IL-1 R4,Fit-1,DER4

Ser27-Ala337

Ser27-Ala337 (Val192Ala)

Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

ST2, also called IL-1 R4, is an Interleukin-1 receptor family glycoprotein that plays a role in Th2 immune responses. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes. This receptor is very similar to the IL-1 receptor type I and the IL-18 receptor α chain in that ST2 also has three extracellular Ig domains and an intracellular Toll domain. ST2 binds IL-33, enhances inflammatory cytokines by activating nuclear factor-κB (NF-κB) and mitogen activated protein (MAP) kinases. ST2 exists as either a membrane bound form (ST2L) or as a soluble form (sST2). ST2L acts as a transmembrane signalling receptor for IL-33 by mediating the effect of IL-33 on the inflammatory process, while sST2 can suppress IL-33 activity.