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TEM1/CD248 Protein, Canine, Recombinant (His)

Catalog No. TMPK-01321

TEM1/CD248 Protein, Canine, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 72.72 kDa and the accession number is XP_025302002.1.

TEM1/CD248 Protein, Canine, Recombinant (His)

TEM1/CD248 Protein, Canine, Recombinant (His)

Catalog No. TMPK-01321
TEM1/CD248 Protein, Canine, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 72.72 kDa and the accession number is XP_025302002.1.
Pack SizePriceAvailabilityQuantity
100 μg$4877-10 days
500 μg$1,9507-10 days
1 mg$3,2507-10 days
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Product Information

Biological Activity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
TEM1/CD248 Protein, Canine, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 72.72 kDa and the accession number is XP_025302002.1.
Species
Canine
Expression System
HEK293 Cells
TagC-His
Accession NumberXP_025302002.1
Synonyms
TEM1,Endosialin,CD248 molecule,CD248,CD164L1,CD164 sialomucin-like 1
Construction
Gln18-Gly687
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight72.72 kDa (predicted). Due to glycosylation, the protein migrates to 120-140 kDa based on Tris-Bis PAGE result.
Endotoxin< 1 EU/μg by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
Colorectal cancer (CRC) is one of the most common cancers worldwide usually diagnosed in the advanced stage. The serum concentration of tumor endothelial marker 1 (TEM1) was measured and correlated with clinicopathological features to evaluate whether TEM1 might serve as a biomarker for early CRC diagnosis, progression, and prognosis. TEM1 can act as a potential diagnostic, progression, and prognostic serum biomarker for patients with CRC; TEM1 might be a good supplement for commonly used markers CEA and Ca 19-9.

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