Thrombopoietin Protein, Mouse, Recombinant (His)

Catalog No. TMPY-00939

Thrombopoietin Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37 kDa and the accession number is P40226-1.

Thrombopoietin Protein, Mouse, Recombinant (His)

Catalog No. TMPY-00939

Thrombopoietin Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37 kDa and the accession number is P40226-1.

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100 μg	$397	In Stock
200 μg	$673	7-10 days

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Product Information

Biological Activity	Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 1.6-6.4 ng/mL.
Description	Thrombopoietin Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37 kDa and the accession number is P40226-1.
Species	Mouse
Expression System	HEK293 Cells
Tag	N-His
Accession Number	P40226-1
Synonyms	Tpo,thrombopoietin,Mpllg,Ml,Mgdf
Construction	A DNA sequence encoding the mouse THPO (NP_033405.1) precursor (Met 1-Thr 356) with a N-terminal polyhistidine tag was expressed. Predicted N terminal: Ser 22
Protein Purity	> 90 % as determined by SDS-PAGE
Molecular Weight	37 kDa (predicted); 80-90 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Thrombopoietin (TPO or THPO), also known as myeloproliferative leukemia virus ligand (c-Mpl), is a hematopoietic growth factor belonging to the EPO/TPO family. The thrombopoietin protein is produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. Thrombopoietin protein stimulates both proliferation of progenitor megakaryocytes and their maturation to platelet-producing megakaryocytes, and also accelerates the recovery of platelets. Thrombopoietin protein is involved in cardiovascular disease as it regulates megakaryocyte development and enhances platelet adhesion/aggregation. It has been identified that surface c-MPL, the receptor for thrombopoietin protein, binds to the ligand and mediates the action.