1. Loaded Human TPBG Antibody (5G9) (Human IgG1) on Protein-A Biosensor, can bind Human TPBG (C-mFc) with an affinity constant of 8.75 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Human TPBG (C-mFc) at 2μg/ml (100 μl/well) can bind Human TPBG Antibody (5G9) (Human IgG1) . The ED50 of Human TPBG Antibody (5G9) (Human IgG1) is 1.57 ng/ml. (Regularly tested)

5T4/TPBG Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with C-mFc tag. The predicted molecular weight is 80-110 KDa and the accession number is Q13641.

Human

HEK293 Cells

WAIF1 Protein,TPBG,M6P1 Protein,5T4AG Protein,5T4 Protein

Ser32-Ser355

Ser32-Ser355

Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Trophoblast glycoprotein, also known as TPBG and 5T4, it belongs to the LRR family of proteins. The C-terminus of LRR N-terminal cap (LRRNT) and LRR 1 are essential for the inhibition of the Wnt signaling pathway. TPBG may function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. It expressed by all types of trophoblasts as early as 9 weeks of development. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.