1. Loaded Human TPBG Antibody (5G9) (Human IgG1) on Protein-A Biosensor, can bind Biotinylated Human 5T4 (C-Avi-6His) with an affinity constant of 9.29 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Human TPBG Antibody (5G9) (Human IgG1) at 2μg/ml (100 μl/well) can bind Biotinylated Human TPBG-Avi-His. The ED50 of Human Biotinylated TPBG-Avi-His is 0.13 ng/ml. (Regularly tested)

5T4/TPBG Protein, Human, Recombinant (Avi & His), Biotinylated is expressed in HEK293 mammalian cells with C-Avi-6xHis tag. The predicted molecular weight is 60-90 KDa and the accession number is Q13641.

Human

HEK293 Cells

5T4,M6P1,WAIF1,5T4AG,TPBG

Ser32-Ser355

Ser32-Ser355

Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Trophoblast glycoprotein, also known as TPBG and 5T4, it belongs to the LRR family of proteins. The C-terminus of LRR N-terminal cap (LRRNT) and LRR 1 are essential for the inhibition of the Wnt signaling pathway. TPBG may function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. It expressed by all types of trophoblasts as early as 9 weeks of development. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.