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Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $347 | 7-10 days | |
500 μg | $1,400 | 7-10 days | |
1 mg | $2,330 | 7-10 days |
Biological Information | 1. Immobilized Human Transferrin R, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Transferrin R Antibody, hFc Tag with the EC50 of 26.5ng/ml determined by ELISA (QC Test).
2. Human Transferrin R, hFc Tag captured on CM5 Chip via Anti-Fc Antibody can bind Human Transferrin, His Tag with an affinity constant of 0.1μM as determined in SPR assay. |
Description | The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status. Transferrin R/CD71 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 101.8 kDa and the accession number is P02786-1. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-hFc |
Accession Number | P02786-1 |
Synonyms | sTfR,CD71,TfR1,TFR,p90,TFRC,TR,T9 |
Construction | Cys89-Phe760 |
Protein Purity | > 95% as determined by Tris-Bis PAGE |
Molecular Weight | 101.8 kDa (predicted). Due to glycosylation, the protein migrates to 105-115 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status. |
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