Immobilized Recombinant Human/Mouse/Rat/Cynomolgus/Canine BDNF Protein at 2 μg/mL (100 μL/well) can bind TrkB Protein, Human, Recombinant (His), Biotinylated (Cat#TMPY-06997), the EC50 is 10-30 ng/mL.

TrkB Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 Cells. The accession number is Q16620-2.

Human

HEK293 Cells

trk-B,TRKB,OBHD,GP145-TrkB,EIEE58,DEE58

A DNA sequence encoding the extracellular domain (Met1-His430) of human TrkB / NTRK2 (NP_001007098.1) precursor was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.

≥ 95% as determined by SDS-PAGE.

Please refer to the lot-specific COA.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) is a single transmembrane catalytic receptor with intracellular tyrosine kinase activity. TrkB/NTRK2 is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. TrkB tyrosine kinase (TrkB) or NTRK2 is coupled to the Ras, Cdc42/Rac/RhoG, MAPK, PI3-K, and PLCgamma signaling pathways. There are four members of the Trk family; TrkA, TrkB, and TrkC and a related p75NTR receptor. Each family member binds different neurotrophins with varying affinities. TrkB/NTRK has the highest affinity for brain-derived neurotrophic factor (BDNF) and is involved in neuronal plasticity, long-term potentiation, and apoptosis of CNS neurons. Other neurotrophins includenerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. TrkB/NTRK is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signaling through this kinase leads to cell differentiation. Mutations in TrkB/NTRK have been associated with obesity and mood disorders.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy