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UCHL1 Protein, Rat, Recombinant (His)

Catalog No. TMPY-02331

UCHL1 Protein, Rat, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 26.2 kDa and the accession number is Q00981.

UCHL1 Protein, Rat, Recombinant (His)

UCHL1 Protein, Rat, Recombinant (His)

Catalog No. TMPY-02331
UCHL1 Protein, Rat, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 26.2 kDa and the accession number is Q00981.
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50 μg570 €7-10 days
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Product Information

Biological Activity
Measured by the hydrolysis of UbiquitinAMC. The specific activity is >100 pmoles/min/μg.
Description
UCHL1 Protein, Rat, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 26.2 kDa and the accession number is Q00981.
Species
Rat
Expression System
E. coli
TagN-His
Accession NumberQ00981
Synonyms
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Construction
A DNA sequence encoding the rat UCHL1 (Q00981) (Glu 2-Ala 223) was fused with a polyhistidine tag at the N-terminus. Predicted N terminal: Met
Protein Purity
> 97 % as determined by SDS-PAGE
Molecular Weight26.2 kDa (predicted); 28 kDa (reducing conditions)
EndotoxinPlease contact us for more information.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinson's, Alzheimer's, and Huntington's disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons.

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