10-Octadecylacridine orange (bromide) serves as a versatile dye, instrumental in both scientific and industrial applications. Within the realm of biological experiments, this dye facilitates the observation and analysis of cell structures, enables tracking of biomolecules, assists in cell function evaluation, aids in distinguishing cell types, detects biomolecules, supports tissue pathology studies, and monitors microorganisms. Its use extends beyond basic research into a broad spectrum of clinical diagnostics. Additionally, the dye is prevalent in traditional sectors like textile dyeing and in innovative areas including functional textile processing, food pigments, and dye-sensitized solar cells.