QCan Tween-80 be used instead of Tween-20?
ATween-20 is not recommended. Tween-80 has better tolerance and has been used as a tool to assess the behavioral effects of experimental drugs and toxins without significant side effects.
Reference: Castro CA, Hogan JB, Benson KA, Shehata CW, Landauer MR. Behavioral effects of vehicles: DMSO, ethanol, Tween-20, Tween-80, and emulphor-620. Pharmacol Biochem Behav. 1995 Apr;50(4):521-6.
QHow to choose the appropriate solvent?
ACommon solvents include DMSO, water, ethanol, etc. Please select the appropriate solvent according to the solubility reference. If the inhibitor can be dissolved in DMSO, it is recommended to use freshly opened DMSO without moisture absorption. If the inhibitor can be dissolved in water, solvents like sterile water, physiological saline, sterile PBS ahd culture medium are recommended.
QWhat's the meaning of "< 1 mg/mL refers to the slightly soluble or insoluble"?
AIt means that if the solubility is less than 1 mg/mL
QCan inhibitors be used for cell experiments?
AYes, our inhibitors can be used for cell/in vitro experiments. However, some compounds may not have literature supporting their use in cell experiments. In such cases, we cannot guarantee efficacy.
QWhat should I do if the IC50 obtained for cell killing are significantly different from that presented on the your website?
AThe IC50 you obtain refers to the cell proliferation inhibition assay (half maximal inhibitory concentration), which is different in meaning from the IC50 on our website. The IC50 often refers to the inhibition rate of the target without cell experiments, which is generally determined through kinase or protein purification experiments, and is commonly reported in nanomolar concentrations. However, the IC50 obtained from cell proliferation inhibition assays refers to the half-maximal lethality of the cells, which involves cellular metabolism and penetration, so the concentration is usually higher.
Furthermore, the effect of the same compound on different cell models varies. It is recommended to try increasing the incubation volume and extending the incubation time.