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3x DYKDDDDK Tag
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Catalog No. T83343Cas No. 921765-76-6
3x FLAG-DYKDDDDK Tag is a versatile tool utilized for studying protein function and localization when specific antibodies (Abs) are unavailable. It is frequently employed in its 3x FLAG format to facilitate the purification of proteins present in low quantities [1] [2].
3x DYKDDDDK Tag
😃Good
Catalog No. T83343Cas No. 921765-76-6
3x FLAG-DYKDDDDK Tag is a versatile tool utilized for studying protein function and localization when specific antibodies (Abs) are unavailable. It is frequently employed in its 3x FLAG format to facilitate the purification of proteins present in low quantities [1] [2].
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 5 mg | Inquiry | Backorder | |
| 50 mg | Inquiry | Backorder |
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Product Introduction
Bioactivity
Chemical Properties
| Description | 3x FLAG-DYKDDDDK Tag is a versatile tool utilized for studying protein function and localization when specific antibodies (Abs) are unavailable. It is frequently employed in its 3x FLAG format to facilitate the purification of proteins present in low quantities [1] [2]. |
| In vitro | HEK293 cells, transiently transfected with plasmids encoding HARS, were collected and lysed using CelLytic M buffer supplemented with mammalian protease inhibitor cocktail for 20 minutes at 4°C. |
| Molecular Weight | 3002.88 |
| Formula | C123H176N30O58 |
| Cas No. | 921765-76-6 |
Storage & Solubility Information
| Storage | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . A total of 10 animals were administered, and the formula you used is 5% 
DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.Preparation method for in vivo formula: Take 50 μL DMSO main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O mix well and clarify
main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O mix well and clarifyFor Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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