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4-Cyclohexyl-1-phenylthiosemicarbazide

Catalog No. T1727Cas No. 27421-91-6

4-Cyclohexyl-1-phenylthiosemicarbazide is employed as an intermediate.

4-Cyclohexyl-1-phenylthiosemicarbazide

4-Cyclohexyl-1-phenylthiosemicarbazide

Purity: 99.29%
Catalog No. T1727Cas No. 27421-91-6
4-Cyclohexyl-1-phenylthiosemicarbazide is employed as an intermediate.
Pack SizePriceAvailabilityQuantity
1 mg$35In Stock
2 mg$48In Stock
5 mg$72In Stock
10 mg$93In Stock
25 mg$168In Stock
50 mg$249In Stock
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Purity:99.29%
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Product Introduction

Bioactivity
Description
4-Cyclohexyl-1-phenylthiosemicarbazide is employed as an intermediate.
Kinase Assay
Recombinant ROCK-I, ROCK-II, PKN, or citron kinase is expressed in HeLa cells as Myc-tagged proteins by transfection using Lipofectamine, and is precipitated from the cell lysates by the use of 9E10 monoclonal anti-Myc antibody coupled to G protein-Sepharose. Recovered immunocomplexes are incubated with various concentrations of [32P]ATP and 10 mg of histone type 2 as substrates in the absence or presence of various concentrations of either Y-27632 or Y-30141 at 30°C for 30 min in a total volume of 30 μL of the kinase buffer containing 50 mM HEPES-NaOH, pH 7.4, 10 mM MgCl2, 5 mM MnCl2, 0.02% Briji 35, and 2 mM dithiothreitol. PKCa is incubated with 5 μM [32P]ATP and 200 μg/mL histone type 2 as substrates in the absence or presence of various concentrations of either Y-27632 or Y-30141 at 30°C for 10 min in a kinase buffer containing 50 mM Tris-HCl, pH 7.5, 0.5 mM CaCl2, 5 mM magnesium acetate, 25 μg/mL phosphatidyl serine, 50 ng/mL 12-O-tetradecanoylphorbol-13-acetate and 0.001% leupeptin in a total volume of 30 μL. Incubation is terminated by the addition of 10 μL of 43 Laemmli sample buffer. After boiling for 5 min, the mixture is subjected to SDS-polyacrylamide gel electrophoresis on a 16% gel. The gel is stained with Coomassie Brilliant Blue, and then dried. The bands corresponding to histone type 2 are excised, and the radioactivity is measured[1].
Chemical Properties
Molecular Weight249.38
FormulaC13H19N3S
Cas No.27421-91-6
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 10 mM
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM4.0099 mL20.0497 mL40.0994 mL200.4972 mL
5 mM0.8020 mL4.0099 mL8.0199 mL40.0994 mL
10 mM0.4010 mL2.0050 mL4.0099 mL20.0497 mL

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