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4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-Glucopyranoside

🥰Excellent
Catalog No. T37193Cas No. 37067-30-4

4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside is a fluorogenic substrate for β-hexosaminidases. Upon enzymatic cleavage by β-hexosaminidases, 4-methylumbelliferone (4-μU) is released, and its fluorescence quantifies β-hexosaminidase activity. 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high pH (7.12-10.3), respectively, and an emission maximum ranging from 445 to 455 nm, increasing as pH decreases. This compound has been used to quantify β-hexosaminidase activity in serum or leukocytes from patients with GM2 gangliosidoses, such as Tay-Sachs disease, which is characterized by defects in the α subunit of β-hexosaminidase.

4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-Glucopyranoside

4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-Glucopyranoside

🥰Excellent
Catalog No. T37193Cas No. 37067-30-4
4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside is a fluorogenic substrate for β-hexosaminidases. Upon enzymatic cleavage by β-hexosaminidases, 4-methylumbelliferone (4-μU) is released, and its fluorescence quantifies β-hexosaminidase activity. 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high pH (7.12-10.3), respectively, and an emission maximum ranging from 445 to 455 nm, increasing as pH decreases. This compound has been used to quantify β-hexosaminidase activity in serum or leukocytes from patients with GM2 gangliosidoses, such as Tay-Sachs disease, which is characterized by defects in the α subunit of β-hexosaminidase.
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Product Introduction

Bioactivity
Description
4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside is a fluorogenic substrate for β-hexosaminidases. Upon enzymatic cleavage by β-hexosaminidases, 4-methylumbelliferone (4-μU) is released, and its fluorescence quantifies β-hexosaminidase activity. 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high pH (7.12-10.3), respectively, and an emission maximum ranging from 445 to 455 nm, increasing as pH decreases. This compound has been used to quantify β-hexosaminidase activity in serum or leukocytes from patients with GM2 gangliosidoses, such as Tay-Sachs disease, which is characterized by defects in the α subunit of β-hexosaminidase.
Chemical Properties
Molecular Weight379.36
FormulaC18H21NO8
Cas No.37067-30-4
Relative Density.1.4163 g/cm3 (Estimated)
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 30 mg/mL (79.08 mM), Sonication is recommended.
DMF: 5 mg/mL (13.18 mM), Sonication is recommended.
Solution Preparation Table
DMF/DMSO
1mg5mg10mg50mg
1 mM2.6360 mL13.1801 mL26.3602 mL131.8009 mL
5 mM0.5272 mL2.6360 mL5.2720 mL26.3602 mL
10 mM0.2636 mL1.3180 mL2.6360 mL13.1801 mL
DMSO
1mg5mg10mg50mg
20 mM0.1318 mL0.6590 mL1.3180 mL6.5900 mL
50 mM0.0527 mL0.2636 mL0.5272 mL2.6360 mL

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Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
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