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5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside

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Catalog No. T85479Cas No. 15548-60-4

5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside, a chromogenic substrate, is widely utilized in molecular biology for β-galactosidase activity detection, especially in gene expression and reporter gene analysis. When cleaved by β-galactosidase, it yields a blue precipitate visible through microscopy or alternative detection techniques. Known for its high sensitivity and specificity, it remains a valuable asset in molecular biology research.

5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside

5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside

😃Good
Catalog No. T85479Cas No. 15548-60-4
5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside, a chromogenic substrate, is widely utilized in molecular biology for β-galactosidase activity detection, especially in gene expression and reporter gene analysis. When cleaved by β-galactosidase, it yields a blue precipitate visible through microscopy or alternative detection techniques. Known for its high sensitivity and specificity, it remains a valuable asset in molecular biology research.
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Product Introduction

Bioactivity
Description
5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside, a chromogenic substrate, is widely utilized in molecular biology for β-galactosidase activity detection, especially in gene expression and reporter gene analysis. When cleaved by β-galactosidase, it yields a blue precipitate visible through microscopy or alternative detection techniques. Known for its high sensitivity and specificity, it remains a valuable asset in molecular biology research.
In vitro
5-Bromo-4-chloro-3-indolyl β-D-Glucopyranoside is a biochemical reagent used as a biomaterial or organic compound in life sciences research.
Chemical Properties
Molecular Weight408.63
FormulaC14H15BrClNO6
Cas No.15548-60-4
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

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TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
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