8-Azido-ATP is a nucleotide analog that demonstrates photo-reactivity properties. It offers utility in the precise labeling and identification of proteins, particularly those involved in DNA-dependent RNA polymerase activity.

8-Azido-ATP (azido-ATP) serves as an effective tool in identifying viral RNA polymerase by inhibiting transcriptional activity in rotavirus particles upon UV exposure. This compound reduces viral transcription in a dose-dependent fashion. Additionally, 8-Azido-ATP exhibits a reduced potency in blocking Kir6.2ΔC26 currents compared to ATP, with a half-maximal inhibition (Ki) observed at 2.8 ± 0.4 mM for 8-azido-ATP versus 172 ± 7 mM for ATP, indicating a lower efficacy. The Hill coefficients for 8-azido-ATP and ATP are 0.9 ± 0.2 and 1.3 ± 0.1, respectively, suggesting differences in binding affinity.