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NucPE1

NucPE1
NucPE1, a nuclear-localized fluorescent hydrogen peroxide, is specifically localized to cellular nuclei without appended targeting moieties.
Catalog No. T19042Cas No. 1404091-23-1

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NucPE1

Catalog No. T19042Alias Nuclear Peroxy Emerald 1Cas No. 1404091-23-1

NucPE1, a nuclear-localized fluorescent hydrogen peroxide, is specifically localized to cellular nuclei without appended targeting moieties.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Pack SizePriceAvailabilityQuantity
5 mg$4477-10 days
10 mg$8147-10 days
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Product Introduction

Bioactivity
Description
NucPE1, a nuclear-localized fluorescent hydrogen peroxide, is specifically localized to cellular nuclei without appended targeting moieties.
In vitro
NucPE1 exhibits two primary absorptions in the visible region (λabs=468 nm, ε=27,300 M-1cm-1; λabs=490 nm, ε=26,000 /(M·cm)) and a weak emission (λem=530 nm, Φ=0.117). The reaction of NucPE1 with H2O2 induces a fluorescence increase by converting it to fluorophore NucPE1, which shows a major absorption at 505 nm (ε=19,100 /(M·cm)) and enhanced emission (λem=530 nm, Φ=0.626). NucPE1 selectively accumulates in the nuclei of various mammalian cell lines and in whole model organisms like C. clegans, where it can detect subcellular H2O2 flux changes.
In vivo
NucPE1 imaging reveals a reduction in nuclear H2O2 levels in worms overexpressing sir-2.1 compared to wildtype congeners.
AliasNuclear Peroxy Emerald 1
Chemical Properties
Molecular Weight483.36
FormulaC29H30BNO5
Cas No.1404091-23-1
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 25 mg/mL (51.72 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.0689 mL10.3443 mL20.6885 mL103.4426 mL
5 mM0.4138 mL2.0689 mL4.1377 mL20.6885 mL
10 mM0.2069 mL1.0344 mL2.0689 mL10.3443 mL
20 mM0.1034 mL0.5172 mL1.0344 mL5.1721 mL
50 mM0.0414 mL0.2069 mL0.4138 mL2.0689 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
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