QHow to store the inhibitor solutions?
AAfter the stock solution is prepared, it is typically stored at -80°C and can be kept for over 1 year. It is recommended to aliquot to avoid repeated freeze-thaw cycles. For frequent use, it can be stored at 4°C for over a week.
QHow to dilute working solutions?
ACalculate the dilution factor and volume of the stock solution needed based on the concentration of your working solution. It is recommended to use solvents such as water, saline solution, PBS, or culture medium for dilution. For cell experiments, culture medium is suitable.
Slowly add the appropriate solvent to the stock solution until you reach the desired concentration of your working solution. Gentle vortexing or pipetting can help mix the solution evenly.
Most of our compounds are lipophilic, and precipitation may occur when using PBS or culture medium to dilute the working solution. It is normal and the product can usually be completely dissolved by sonication.
It is recommended to prepare and use the working solution immediately after dilution
Qwhat frequency should sonication be used?
AIt is recommended to use the conventional ultrasound cleaning frequency
QCan inhibitors be used for animal experiments?
AYes, our inhibitors can be used for animal/in vivo experiments. However, some compounds may not have literature supporting their use in animal experiments. In such cases, we cannot guarantee efficacy.
QWhat are the common methods for dissolving compounds in animal experiments?
AFirst, you need to confirm the dosage and administration route. For specific products, it is recommended to refer to literatures that cite our product. If there are no relevant references and the compound dissolves well in DMSO, we recommend a general formula: 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline/PBS/ddH2O. The solvents should be added sequentially, and each solvent should be added after ensuring as much dissolution as possible. For normal mice, keep the DMSO concentration below 10%, and for nude mice and mice with weak tolerance, the DMSO concentration should be kept below 2%. The ratio of PEG300 and Tween-80 can be adjusted accordingly based on whether the solution is clear or not. Other co-solvents can also be used.
The above formula is for reference only. Please adjust according to different conditions. It is recommended to first test a small amount of compound with the formula before preparing a large quantity. Additionally, sonication or heating can be used to accelerate dissolution.
For intraperitoneal injection, it is preferable to use salt forms of compounds as they have higher solubility. If the dosage is large, suspension may be used for intraperitoneal administration.
For oral gavage, especially with larger doses, it is recommended to prepare a homogeneous suspension using 0.5% CMC-Na.