Acridine Orange 10-Nonyl Bromide is a cardiolipin fluorescent probe (λex: 489 nm, λem: 525 nm).

Acridine Orange 10-Nonyl Bromide serves as a fluorescent probe specifically for cardiolipin, exhibiting excitation (λex) at 489 nm and emission (λem) at 525 nm, enabling the quantification of cardiolipin in isolated mitochondria[1]. Interaction with cardiolipin alters its excitation and emission wavelengths from 496 and 525 nm to 450 and 640 nm, respectively. Moreover, the addition of varying levels of cardiolipin (0 to 30 μM) and other acidic phospholipids to Acridine Orange 10-Nonyl Bromide (45 μM) in thin-walled vesicles results in measurable changes in the red fluorescence emission at 640 nm, reflective of the liposome composition[2].