APE-IN-1 can be used for the early diagnosis and drug research of Alzheimer's disease. APE-IN-1 is a potent Asparagine endopeptidase (AEP) inhibitor with IC 50 value of 89 nM [1].

APE-IN-1, when applied in concentrations ranging from 5-500 nM for a 24-hour period, does not influence the viability of L929 normal fibroblasts across the tested concentration spectrum [1]. This was determined using a cell viability assay conducted on L929 cells at varying concentrations of 5, 10, 50, 100, and 500 mM over an incubation period of 24 hours, indicating no detrimental effect on cell viability even at higher doses.