Anti-infective agent 5 (compound 74) is an orally active inhibitor of Trypanosoma cruzi with an IC50 value of 0.10 μM, demonstrating efficacy in reducing parasite burden in vivo and holding promising potential for infection research. [1]

The anti-infective agent 5, administered at concentrations ranging from 0.1 nM to 100 μM for 48 hours, exhibits antitrypanosomal effects against T. cruzi amastigotes within 3T3 cells, achieving an IC50 of 0.10 μM [1]. Furthermore, in cytotoxicity assays conducted on the 3T3 cell line under the same conditions, the compound demonstrated a CC50 value of over 37 μM, indicating its activity and potential selectivity towards the pathogen while maintaining a lower toxicity profile towards the host cells [1].

The anti-infective agent 5, administered orally at a dosage of 50 mg/kg twice daily for five days, significantly reduced the parasite burden in a BALB/c mouse model infected with bioluminescent T. cruzi CL Brener parasites. This treatment resulted in a greater than 98-99% decrease in parasite levels without causing notable adverse effects in the mice [1].