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Anti-EFNB1 Monoclonal Antibody-AF555
Catalog No. TMAZ-0223A5
Alias Ephrin-B1, EPH-related receptor tyrosine kinase ligand 2, ELK ligand, EFL-3
Anti-EFNB1 Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting EFNB1. Anti-EFNB1 Monoclonal Antibody-AF555 can be used in ELISA, FACS.
Anti-EFNB1 Monoclonal Antibody-AF555
Anti-EFNB1 Monoclonal Antibody-AF555
Catalog No. TMAZ-0223A5Alias Ephrin-B1, EPH-related receptor tyrosine kinase ligand 2, ELK ligand, EFL-3
Anti-EFNB1 Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting EFNB1. Anti-EFNB1 Monoclonal Antibody-AF555 can be used in ELISA, FACS.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μg | $360 | 12 days | |
| 50 μg | $600 | 12 days | |
| 100 μg | $900 | 12 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-EFNB1 Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting EFNB1. Anti-EFNB1 Monoclonal Antibody-AF555 can be used in ELISA, FACS. |
| Alias | Ephrin-B1, EPH-related receptor tyrosine kinase ligand 2, ELK ligand, EFL-3 |
| Ig Type | Rabbit monoclonal IgG |
| Reactivity | Human |
| Verified Activity | Flow cytometry analysis of EFNB1 overexpressed 327F cells with TMAZ-0223A5, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line). |
| Application | ELISA, FACS |
| Recommended Dose | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Single-pass type I membrane |
| Construction | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of EFNB1 protein. |
| Purification | Protein A Affinity Purified |
| Appearance | Liquid |
| Formulation | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| Purity | > 95% as determined by SDS-PAGE. |
| Research Background | Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development (PubMed: 7973638, PubMed: 8070404). Binding to Eph receptors residing on adjacent cells leads to contact-dependent bidirectional signaling into neighboring cells (PubMed: 7973638, PubMed: 8070404). Shows high affinity for the receptor tyrosine kinase EPHB1/ELK (PubMed: 7973638, PubMed: 8070404). Can also bind EPHB2 and EPHB3 (PubMed: 8070404). Binds to, and induces collapse of, commissural axons/growth cones in vitro (By similarity). May play a role in constraining the orientation of longitudinally projecting axons (By similarity) |
| Conjucates | AF555 |
| Others Formats | Biotin/APC/PE/AF488/AF647 |
| Antibody Types Available | 1 |
| Immunogen | EFNB1 |
| Antigen Species | Human |
| Gene ID | 1947 |
| Uniprot ID | |
| Biology Area | Cytokines |
| Molecular Weight | 150 kDa |
Stability & Storage
| Stability & Storage | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| Transport | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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