Antibacterial agent 121 (Compound 10) exhibits antibacterial properties against mycobacterial strains and possesses anti-inflammatory activity, making it a promising candidate for Tuberculosis (TB) research [1].

Antibacterial agent 121 (0.2-200 μM; 24 h) inhibits M. tuberculosis H37Rv growth in bacterial culture [1] and reduces NO production in LPS-stimulated RAW 264.7 cell cultures with an IC 50 value of 4.1 μM [1]. It also inhibits TNF-α and IL-1β production in LPS-stimulated macrophages (6.25-200 μM; 24 h) [1] and M. tuberculosis M299 with an MIC 50 value of 24.8 μM (25-100 μM; 4 d) [1]. Cell viability assays showed it inhibited Mtb H37Rv growth with an MIC 50 of 8.6 μM (0.2-200 μM; 24 h) and exhibited IC 50 values of 15.3 μM and 28.4 μM for TNF-α and IL-1β production in RAW 264.7 and J774A.1 macrophages, respectively (6.25-200 μM; 24 h) [1].