Antitumor agent-23 exhibits potent antitumoral activity, as demonstrated by its GI50 values of 38, 48, 5, 27, 80, and 28 nM against the lymphoma cell lines GRANTA-519, KARPAS-422, KARPAS-299, RAMOS, DAUDI, and RAJI respectively.

Antitumor agent-23, ranging from 0.001 to 100 μM over five days, effectively inhibits the proliferation of various lymphoma cell lines[1]. In HPV-positive cervical cancer cells, concentrations between 2 and 70 nM over 72 hours significantly upregulate p53 protein levels and induce acetylation at lysine 382 of p53 (K382Ac-p53)[1]. Detailed Cell Proliferation Assay findings reveal GI50 values of 38, 48, 5, 27, 80, and 28 nM for GRANTA-519, KARPAS-422, KARPAS-299, RAMOS, DAUDI, and RAJI cell lines, respectively, over a five-day period[1]. Western Blot Analysis conducted on the HPV16-positive cervical cancer cell line (CaSki) demonstrated noticeable p53 protein expression enhancement starting at 7 nM and noticeable acetylation at p53 lysine 382 starting at 20 nM over a 72-hour incubation period[1].