Antitumor agent-68 is a potent tubulin inhibitor exhibiting significant antitumor activity, with IC 50 values of 3.6 μM for HeLa and 3.8 μM for MCF-7 cells. It also demonstrates good scavenging activity of ROS and DPPH radicals in a dose-dependent manner [1].

Antitumor agent-68 (Compound 7) demonstrates anticancer activity with IC50 values of 3.6 and 3.8 μM for HeLa and MCF-7 cells, respectively [1]. At 10 μΜ for 24 hours, it reduces Men-induced ROS production by 3-fold in 3T3-L1 cells and has an EC50 of 8.30 μg/mL for DPPH radical scavenging [1]. Cell Viability Assay [1]: Cell Lines: HeLa, MCF-7, 3T3-L1; Concentration: 10 μM; Incubation Time: 24 hours; Result: Demonstrated anticancer activity by hindering tubulin polymerization, reducing ROS production, and scavenging DPPH radicals.