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Autophagy-IN-2

Catalog No. T60749 CAS 2755454-90-9

Autophagy-IN-2 (Compound 7h) is an inhibitor of autophagic flux which induces cancer cell apoptosis and can be used for the research of triple-negative breast cancer [1].

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Autophagy-IN-2, CAS 2755454-90-9

Pack Size	Availability	Price/USD
25 mg	6-8 weeks	$ 1,520.00
50 mg	6-8 weeks	$ 1,980.00
100 mg	6-8 weeks	$ 2,500.00

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This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.

Biological Description

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Description	Autophagy-IN-2 (Compound 7h) is an inhibitor of autophagic flux which induces cancer cell apoptosis and can be used for the research of triple-negative breast cancer [1].
In vitro	Autophagy-IN-2 (Compound 7h), across a concentration range of 0-200 μM for 48 hours, exhibits anti-viability effects on various cancer cell lines, as evidenced by its IC 50 values across a panel including MDA-MB-231, MDA-MB-468, and others. At lower concentrations (0-20 μM) and varying time points up to 48 hours, this compound disrupts autophagic flux and DNA repair mechanisms by decreasing chromatin ubiquitination, linked to a p62-dependent process. Additionally, Autophagy-IN-2 triggers cell cycle arrest in the S-phase and initiates mitochondrial-dependent intrinsic apoptosis. Western blot analysis reveals a dose and time-dependent increase in LC3B-II, p62 accumulation, and DNA damage markers in treated TNBC cells, alongside modifications in cell cycle and apoptosis-related proteins. Cell cycle and apoptosis assays further confirm the compound's ability to induce S-phase arrest and significantly enhance apoptosis, particularly late-phase, in a dose-dependent manner in TNBC cells.
In vivo	Autophagy-IN-2 (Compound 7h) administered intraperitoneally (i.p.) at doses of 5 and 15 mg/kg every three days for three weeks has been observed to dose-dependently suppress tumor growth in six-week-old female NOD/SCID mice with MDA-MB-231 xenografts. This treatment led to a dose-dependent inhibition of human TNBC tumor growth and induced a concentration-dependent increase in the expression of LC3B-II, p62, γH2AX, and PARP.

Molecular Weight	309.37
Formula	C17H19N5O
CAS No.	2755454-90-9

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Powder: -20°C for 3 years | In solvent: -80°C for 1 year

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Keywords

Autophagy-IN-2 2755454-90-9 inhibitor inhibit

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.