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BCECF

Name: BCECF
Brand: TargetMol
SKU: T18922
Price: 222 USD
Availability: InStock
Rating: 4.5 (1 reviews)

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Catalog No. T18922Cas No. 85138-49-4

Alias 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein

BCECF is a fluorescent probe commonly used to measure pH. It is a dual-excitation ratiometric pH indicator with a pKa of ~6.98. Measurements of pH are made by determining the ratio of emission intensity, detected at 535 nm when excited at 490 nm versus the emission intensity when excited at 440 nm.

BCECF

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Catalog No. T18922Alias 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluoresceinCas No. 85138-49-4

Pack Size	Price	Availability
500 μg	$93	35 days
1 mg	$163	35 days
5 mg	$578	35 days

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All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity

Description	BCECF is a fluorescent probe commonly used to measure pH. It is a dual-excitation ratiometric pH indicator with a pKa of ~6.98. Measurements of pH are made by determining the ratio of emission intensity, detected at 535 nm when excited at 490 nm versus th
In vitro	The staining is seen in the interior of hydrogenosomes in some instances. It is also observed by microscopy that the K+/H+ ionophore nigericin does not inhibit hydrogenosomes loading with BCECF.
Alias	2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein

Chemical Properties

Molecular Weight	1040.892
Formula	C₅₄H₄₀O₂₂
Cas No.	85138-49-4
Relative Density.	1.67g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 62.5 mg/mL (120.09 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	0.9607 mL	4.8036 mL	9.6071 mL	48.0357 mL
5 mM	0.1921 mL	0.9607 mL	1.9214 mL	9.6071 mL
10 mM	0.0961 mL	0.4804 mL	0.9607 mL	4.8036 mL
20 mM	0.0480 mL	0.2402 mL	0.4804 mL	2.4018 mL
50 mM	0.0192 mL	0.0961 mL	0.1921 mL	0.9607 mL
100 mM	0.0096 mL	0.0480 mL	0.0961 mL	0.4804 mL

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

BCECF