QCan Tween-80 be used instead of Tween-20?
ATween-20 is not recommended. Tween-80 has better tolerance and has been used as a tool to assess the behavioral effects of experimental drugs and toxins without significant side effects.
Reference: Castro CA, Hogan JB, Benson KA, Shehata CW, Landauer MR. Behavioral effects of vehicles: DMSO, ethanol, Tween-20, Tween-80, and emulphor-620. Pharmacol Biochem Behav. 1995 Apr;50(4):521-6.
QWhat are the common methods for dissolving compounds in animal experiments?
AFirst, you need to confirm the dosage and administration route. For specific products, it is recommended to refer to literatures that cite our product. If there are no relevant references and the compound dissolves well in DMSO, we recommend a general formula: 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline/PBS/ddH2O. The solvents should be added sequentially, and each solvent should be added after ensuring as much dissolution as possible. For normal mice, keep the DMSO concentration below 10%, and for nude mice and mice with weak tolerance, the DMSO concentration should be kept below 2%. The ratio of PEG300 and Tween-80 can be adjusted accordingly based on whether the solution is clear or not. Other co-solvents can also be used.
The above formula is for reference only. Please adjust according to different conditions. It is recommended to first test a small amount of compound with the formula before preparing a large quantity. Additionally, sonication or heating can be used to accelerate dissolution.
For intraperitoneal injection, it is preferable to use salt forms of compounds as they have higher solubility. If the dosage is large, suspension may be used for intraperitoneal administration.
For oral gavage, especially with larger doses, it is recommended to prepare a homogeneous suspension using 0.5% CMC-Na.
QAfter the inhibitor is dissolved in DMSO to prepare the stock solution, can it be stored at room temperature for one week?
AIt is not recommended to do so. Once the stock solution is prepared
QWhat's the meaning of "< 1 mg/mL refers to the slightly soluble or insoluble"?
AIt means that if the solubility is less than 1 mg/mL
QCan inhibitors be used for animal experiments?
AYes, our inhibitors can be used for animal/in vivo experiments. However, some compounds may not have literature supporting their use in animal experiments. In such cases, we cannot guarantee efficacy.