QWhether sonication is needed for product dissolution? What if I don't have an ultrasound machine?
Asonication can accelerate dissolution. If the compound is not dissolving, sonication is recommended. If you don't have an ultrasound machine, a lower solvent concentration or using less powder are recommended.
QWhether TargetMol inhibitor products are suitable for industrial use?
AThere are various standards for defining the purity, and we do not have fixed purity standard. The purity of most products are above 98% and can be used for control experiments, including cell experiments, animal experiments, general laboratory experiments, and high-throughput screening. Our product purity also meets the industrial standard.
QHow much inhibitor should be added in cell experiments?
AIt is recommended to refer to literatures of experiments conducted using the same model. In addition, it is also necessary to determine the optimal working concentration (concentration gradient) through pre-experiments by constructing a dose-response curve. Similarly, the optimal incubation time (time gradient) should be determined through a time-course experiment. Moreover, the amount of inhibitor used is influenced by various factors, including the accessibility of the target, cell permeability, incubation time, cell type, and others. We recommend determining the initial concentration of the inhibitor by referring to the literatures.
If the Ki or IC50 are reported, it is recommended to start with 5-10 times their reported values to achieve the optimal inhibition of enzyme activity.
If the Ki or IC50 are unknown, the inhibitor concentrations should be tested, and the Ki value can be calculated using Michaelis-Menten kinetics.
Typically, the solvent used for dissolving the inhibitor is used as a control to eliminate the non-specific effects of the solvent.
QWhat is the calculation method for preparing a solution?
AThe calculation method is as follows:
(1) c=n/v=m/M/v
Concentration=amount of substance/volume=mass/molar mass/volume
(2) The solution preparation table is presented in the the product details page on our website. You can add the volume of solvent as referenced.
QHow long does sonication usually take?
AThe sonication time depends on the amount and concentration of the substance