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Bz-Nle-Lys-Arg-Arg-AMC

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Catalog No. T37870Cas No. 863975-32-0
Alias Bz-Nle-Lys-Arg-Arg-AMC, BzNleLysArgArgAMC, Bz Nle Lys Arg Arg AMC

Bz-Nle-Lys-Arg-Arg-AMC is a fluorescent tetrapeptide substrate for the quantitative detection of dengue virus (DV) NS2B-NS3 protease, yellow fever virus (YFV) NS3 protease and Zika virus NS3 protease activities. The rate of release of the cleavage product of the protease, AMC (7-amino-4-methylcoumarin), was observed by hydrolysis reaction to calculate the enzyme activity, with excitation/emission maxima at 340-360/440-460 nm, respectively.

Bz-Nle-Lys-Arg-Arg-AMC

Bz-Nle-Lys-Arg-Arg-AMC

😃Good
Catalog No. T37870Alias Bz-Nle-Lys-Arg-Arg-AMC, BzNleLysArgArgAMC, Bz Nle Lys Arg Arg AMCCas No. 863975-32-0
Bz-Nle-Lys-Arg-Arg-AMC is a fluorescent tetrapeptide substrate for the quantitative detection of dengue virus (DV) NS2B-NS3 protease, yellow fever virus (YFV) NS3 protease and Zika virus NS3 protease activities. The rate of release of the cleavage product of the protease, AMC (7-amino-4-methylcoumarin), was observed by hydrolysis reaction to calculate the enzyme activity, with excitation/emission maxima at 340-360/440-460 nm, respectively.
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Product Introduction

Bioactivity
Description
Bz-Nle-Lys-Arg-Arg-AMC is a fluorescent tetrapeptide substrate for the quantitative detection of dengue virus (DV) NS2B-NS3 protease, yellow fever virus (YFV) NS3 protease and Zika virus NS3 protease activities. The rate of release of the cleavage product of the protease, AMC (7-amino-4-methylcoumarin), was observed by hydrolysis reaction to calculate the enzyme activity, with excitation/emission maxima at 340-360/440-460 nm, respectively.
AliasBz-Nle-Lys-Arg-Arg-AMC, BzNleLysArgArgAMC, Bz Nle Lys Arg Arg AMC
Chemical Properties
Molecular Weight832.99
FormulaC41H60N12O7
Cas No.863975-32-0
SmilesCC=1C=2C(=CC(NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC(=O)C3=CC=CC=C3)CCCC)=O)CCCCN)=O)CCCNC(=N)N)=O)CCCNC(=N)N)=O)=CC2)OC(=O)C1
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMF: 20.00 mg/mL (24.01 mM), Sonication is recommended.
DMSO:PBS (pH 7.2) (1:1): 0.5 mg/mL (0.6 mM), < 0.1 mg/mL (insoluble)
Ethanol: 20.00 mg/mL (24.01 mM), Sonication is recommended.
DMSO: 20.00 mg/mL (24.01 mM), Sonication is recommended.

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Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
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Keywords

Bz-Nle-KRR-AMC
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