CEM114 is a potent chemical compound known as a chemical epigenetic modifier (CEM). It functions by employing CRISPR-Cas9 systems to harness endogenous chromatin machinery, thereby exerting its epigenetic modifying effects.

Treatment with CEM114 at a concentration of 200 nM for 48 hours significantly increases the Green Fluorescent Protein (GFP) signal, indicating that an excess of FK506 can displace CEM114 from the FKBP binding site[1]. In a Cell Viability Assay[1] conducted on HEK 293T cells under the same conditions, a marked activation of the GFP signal was observed.