Chitin synthase inhibitor 2 (compound 2b) is a highly potent inhibitor of chitin synthase, with an IC50 value of 0.09 mM and a K i value of 0.12 mM. This compound exhibits antimicrobial activities in vitro and demonstrates synergistic or additive effects when combined with fluconazole or polyoxin B [1].

Chitin synthase inhibitor 2 (compound 2b) demonstrates effective inhibition of chitin synthase, achieving a 78.3% inhibition percent (IP) at 0.3 mM with an inhibition constant (K i) of 0.12 mM. This compound exhibits significant antifungal properties, with minimum inhibitory concentration (MIC) values of 2, 4, 1, and 2 μg/mL against C. albicans (ATCC 76615), A. fumigatus (GIMCC 3.19), C. albicans (ATCC 90023), and A. flavus (ATCC 16870), respectively. However, at concentrations ranging from 128-512 μg/mL, it shows no antibacterial activity against MRSA (N. 315), S. aureus (ATCC 25923), B. subtilis (ATCC 6633), C. coli (JM 109), P. aeruginosa (ATCC 9027), and B. proteus (ATCC 8427).