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CY5-SE Ditriethylamine salt is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. Excitation (nm):649, Emission (nm): 670.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
1 mg | $55 | In Stock | |
5 mg | $113 | In Stock | |
10 mg | $167 | In Stock | |
25 mg | $329 | In Stock | |
50 mg | $518 | In Stock | |
100 mg | $749 | In Stock |
Description | CY5-SE Ditriethylamine salt is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. Excitation (nm):649, Emission (nm): 670. |
In vitro | Guidelines (This protocol only provides a guideline, and should be modified according to your specific needs). Conjugation of SELP Analogues with Cy5-SE. 1. 1 mL of each 100 μg/mL SELP solution in 100 mM sodium bicarbonate buffer (pH 8.3) is mixed with 10 μL of 1.2 mg/mL Cy5 mono NHS-ester in 10% DMSO and incubates for 2 hours on ice. 2. To quench the reaction, 50 μL of 1 M Tris-HCl (pH 8.0) is added to the reaction solution. 3. Reaction mixtures are loaded onto 1.5 mL Sephadex G-25 columns, and Cy5-conjugated SELPs are eluted by centrifugation for 3 minutes at 1050g[1]. |
Alias | CY5-SE Ditriethylamine salt(146368-14-1 Free base) |
Molecular Weight | 956.26 |
Formula | C49H73N5O10S2 |
Smiles | O=C(CCCCCN1C2=CC=C(S(=O)(O)=O)C=C2C(C)(C)C1=CC=CC=CC3=[N+](CC)C4=C(C3(C)C)C=C(S(=O)([O-])=O)C=C4)ON5C(CCC5=O)=O.CCN(CC)CC.CCN(CC)CC |
Relative Density. | no data available |
Storage | keep away from moisture | Shipping with blue ice. |
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