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DAF-2DA

DAF-2DA
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DAF-2DA

Catalog No. T18966Cas No. 205391-02-2
DAF 2DA is widely used as a probe for NO measurement.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Pack SizePriceAvailabilityQuantity
100 μg$14035 days
250 μg$29635 days
500 μg$57335 days
1 mg$1,07035 days
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Product Introduction

Bioactivity
Description
DAF 2DA is widely used as a probe for NO measurement.
In vitro
Diaminofluorescein in diacetate form (DAF 2DA) is most widely probe for NO measurement. This method is based on application of DAF 2DA which is actively diffused into cells, once taken up by cells cytoplasmic esterases cleave the acetate groups to generate DAF 2DA. The generated DAF-2 can readily react with N2O3, which is an oxidation product of NO to generate the highly fluorescent DAF-2T (triazolofluorescein).
AliasDAF2DA, DAF 2DA, 5,6-Diaminofluorescein diacetat
Chemical Properties
Molecular Weight446.41
FormulaC24H18N2O7
Cas No.205391-02-2
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
1 Enter information below:
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2 Enter the in vivo formulation:
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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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