Dextran Sulfate Sodium Salt (MW 36,000-50,000) is a polyanionic derivative of dextran with a moderate molecular weight and strong permeability through the intestinal epithelium. It is the most widely used agent for inflammatory bowel disease (IBD) modeling and is commonly employed to induce chronic and acute colitis models.

Method: To induce acute colitis, Dextran Sulfate Sodium Salt (MW 36,000-50,000) was administered via drinking water at a concentration of 2.8% (m/V) for 6 consecutive days (from day 0 to day 6).
Results: Compared with the control group, mice in the DSS-induced colitis group exhibited severe clinical symptoms, including significant weight loss, increased DAI scores, and shortened colon length. H&E staining of colon tissue sections from the DSS group revealed inflammatory cell infiltration, goblet and epithelial cell loss, crypt abscesses, thickened and detached muscular layers, and a marked increase in ulcer formation. Additionally, histopathological analysis showed significant colonic tissue damage.